Does VDAC insert into membranes in random orientation?

Spontaneous Folding of Human VDAC into Lipid Membranes is Oriented and Indicates a Partially Uncoupled Mechanism

Supramolecular assembly of VDAC in native mitochondrial outer membranes.

The voltage-dependent anion channel (VDAC) is the most abundant protein in the mitochondrial outer membrane (MOM). Due to its localization, VDAC is involved in a wide range of processes, such as passage of ATP out of mitochondria, and particularly plays a central role in apoptosis. Importantly, the assembly of VDAC provides interaction with a wide range of proteins, some implying oligomerizatio...

An Insight into Ocular Insert

Ocular inserts are sterile preparations, with a thin, multilayered, drugimpregnated, solid or semisolid consistency devices placed into cul-de-sac or conjunctiva sac. They are usually made up of polymeric vehicle containing drug. Ocular drug delivery is one of the most fascinating and challenging tasks being faced by the Pharmaceutical researchers. One of the major barriers of ocular medication...

Large-scale colony screening and insert orientation determination using PCR.

Though many diagnostic PCR methods have been described, few techniques have been developed to rapidly and efficiently screen transformed bacterial colonies for plasmid presence (1). Unfortunately, PCR screening of a large number of colonies is time consuming and requires substantial amounts of polymerase and primers. In this report, we describe a two-step large-scale bacterial colony screening ...

The orientation of nisin in membranes.

Nisin is a 34 residue long peptide belonging to the group A lantibiotics with antimicrobial activity against Gram-positive bacteria. The antimicrobial activity is based on pore formation in the cytoplasmic membrane of target organisms. The mechanism which leads to pore formation remains to be clarified. We studied the orientation of nisin via site-directed tryptophan fluorescence spectroscopy. ...